Composite

Part:BBa_K3221009:Design

Designed by: Qihan Jin   Group: iGEM19_SCU-China   (2019-10-04)


pGAL1-HisG MF-tCYC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 682
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use double enzymes restriction of HindIII and XbaI before adapter ligation.


Source

pGAL1 and tCYC1 is on pYES2 initially. And we get HisG-MF by PCR from CNS3(BBa_K3221001).

References